CHED 841 |
| Charity N. Mosley, William C. Hardy, Lei Wang, Stephanie Gilley, Shuguang Wang, and Hongtao Yu. |
| 2-Phenylbenzimidazole (PBI), an ingredient in sunscreen agents absorbs solar UVB spectrum (280-320nm), can undergo excited state reactions upon light irradiation that may cause adverse effects in humans. Therefore, Salmonella typhimurium strain TA 102 is exposed to PBI or PBI and light concomitantly at various doses. There is no appreciable bacteria death due to the exposure to PBI alone, but causes bacteria death in a dose dependent manner when exposed to both light and PBI. At > 5µM, about half of the bacteria died. The photo-Ames test results indicate that exposure of TA 102 to light and PBI at 0-25µM causes the bacteria to revert, an indication of mutation. Light irradiation of the bacteria mixture with PBI causes the number of revertant colonies to increase in a dose dependent manner, reaching 1200 revertant colonies at > 5µM. This result clearly indicates that PBI is photomutagenic in TA 102. Irradiation of human HaCaT keratinocytes in buffer solutions in the presence of PBI causes the cell to lose its viability while, simultaneously causing DNA fragmentation. |
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Undergraduate Research Poster Session: Environmental Chemistry
2:00 PM-4:00 PM, Monday, 27 March 2006 Georgia World Congress Center -- Ex. Hall B4, Poster
Sci-Mix: LSAMP/REU Poster Session
Division of Chemical Education |